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TISSUE/ORGAN: lung, embryonic
ECACC catalog code: 89111004
Susceptibility to viruses: adenovirus * adenovirus 3 * coronavirus * enterovirus * hepatitis B * poliovirus 1 * poliovirus 2 * poliovirus 3 * vesic. stomatitis (Indiana)
Description: Human embryonic lung, HeLa characteristics
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Isolation of enteroviruses
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. The cells grow in clumps.
Karyotype: 2n = 46
Derived from human embryonic lung. Used for primary isolation of enteroviruses and also found to be sensitive to hepatitis virus. The line shows HeLa markers. This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 5
- Arch Virol 1981;69:117 - PMID: 6171237
- Fed Proc 1960;19:386
- In Vitro 1978;14:469-475 - PMID: 566722
- Nature 1976;259:211-213 - PMID: 1250349
- Science 1961;133:2059
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.