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TUMOR: lymphoma, Hodgkin
DSMZ catalog code: ACC 8
Morphology: single, round to polygonal cells in suspension; heterogeneity in cell size, bi- and polynucleated cells
Description: human Hodgkin's lymphoma established from the pleural effusion of a 37-year-old man with Hodgkin's disease (mixed cellularity progressing to lymphocyte depletion; stage IV at relapse) in 1974 confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.3-1.0 x 106 cells/ml; split 1:2 to 1:4 every second day; seed out at ca. 0.8 x 106 cells/ml at 37C with 5% CO2 saturation density at about 1.0 x 106 cells/ml;
doubling time of about 48 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hypotriploid karyotype with 3.5% polyploidy; 59(56-60)<3n>der(X)der(Y), -X, -1, -1, -2, -2, -3, -4, -5, -5, -6, -8, -9, -9, -10, -10, -10, -11, -12, -13, -14, -15, -15, -16, -16, -18, -19, -20, -21, -22, +25mar;add(1)() * cytogenetics 2 (add(3)(q26),del(4)(q26q31),i(4q),add(5)(p11),add(6)(q14),add(7)(p14),add(7)(q34),del(7)(q31),add(8)(q24),del(8)(p12),del(11)(q23),i(13q),der(13)t(11;13)(p13;q13),add(14)(p12)x2,del(17)(p12),i(18q); apparently typical HD) * cytogenetics 3 (karyotype with extraordinary level of rearrangement including several HSR-bearing markers with ectopic rDNA; resembles published karyotype) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13- CD14- CD15+ CD19- CD21+ CD25- CD30+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse trsnscriptase negative)
- Blood 1986;68:285-292 - PMID: 3013343
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.