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TISSUE/ORGAN: bone marrow
TUMOR: leukemia, acute myeloid
DSMZ catalog code: ACC 14
Morphology: round, single cells in suspension
Description: human acute myeloid leukemia established from the bone marrow of a 59-year-old man with erythroleukemia that developped into acute myeloid leukemia (AML) at relapse in 1977; cells were described to form colonies in soft-agar in response to colony-stimulating factor confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.2-1.0 x 106 cells/ml; split 1:2 to 1:4 every 2-3 days; seed out at ca. 1 x 106 cells/ml at 37C with 5% CO2 maximum density at 1.0-1.5 x 106 cells/ml;
doubling time of ca. 38 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays;
Validated by: cytogenetics 1 (hypodiploid with 5% polyploidy; 45(42-47)<2n>X, -Y, -7, -12, +2m, dup(7)(q12q33), der(8)t(8;?)(p12;?), der(11)t(1;11)(q11;p11), der(17)t(17;?)(p13;?); does not correspond clearly with original karyotype) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13+ CD14- CD15+ CD19- CD33+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CCL 246, ECACC: 86111306
- Blood 1980;56:344-350 - PMID: 6996765
- Blood 1983;62:709-721 - PMID: 6192859
- Science 1978;200:1153-1154 - PMID: 306682
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.