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ECACC catalog code: 90070550
Description: Drosophila embryo
Viruses: See Properties
Depositor: Dr J Sinclair, Dept. of Medicine, Addenbrookes Hospital, Cambridge, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: rhabdovirus Drosophila X, picornavirus Drosophila C
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Schneider's Drosophila medium + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml. Cells may adhere to the tissue culture flask, shake gently to dislodge; 25C; No CO2.
Resistant to osmolarity changes within 225-400 mosmols and to chloride levels 4-80mM; the usual Na/K ratio of 1.6 can be both increased and decreased considerably. Viruses present are non-pathogenic but can be induced when inoculated into a new host.
Research council deposit: Yes;
Price Code: B;
Validated by: isoenzymes (species confirmation)
- Adv Cell Culture 1981;1:125
- Cell 1989;57:1017 - PMID: 2567631
- Genetics & Develop 1989;3:882
- Somat Cell Mol Genet 1985;11:63-70 - PMID: 3919453
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.