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SPECIES: mouse / human
ECACC catalog code: 89101606
Description: Mouse x Human hybrid myeloma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Fusion partner and hybridization studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 1 x 105 to 1 x 106 cells/ml; 5% CO2; 37C.
Derived by a fusion of NS-1 - Ag4 myeloma cells with cells from a human nodular lymphoma. The cells are HPRT- and do not secrete immunoglobulin. They are used as a fusion partner for human B lymphocytes.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1823
- J Immunol Methods 1986;89:61 - PMID: 3084658
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.