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ICLC catalog code: HTL94001
Continuous culture, grown as suspension
round and large cells morphology
SPECIES: human, Caucasian (female, 53 years old)
TISSUE/ORGAN: pleural effusion
TUMOR: leukemia, myelogenous
tumorigenic in nude mice
DEPOSITOR: Dr V. Dessi, San Martino Hospital, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: haemoglobin synthesis; antitumour testing; tumorigenicity; differentiation; pharmacodynamics; cloning; cytotoxicity; cellular effects of hyperthermia; natural killer assay
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Maintain cultures between 1 and 5x105 cells/ml; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR) * isoenzymes (confirmed as human with AST, NP, Pep B)
Availability in cell line catalogues: DSMZ: ACC 10
- Blood 1975;45:321-334 - PMID: 163658
- Blood 1980;56:344-350 - PMID: 6996765
- Clin Haematol 1984;13:461-486 - PMID: 6088146
- J Immunol 1982;129:2504-2510 - PMID: 6982930
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.