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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, chronic, B cell
DSMZ catalog code: ACC 18
Morphology: lymphoblastoid cells growing in clumps in suspension
TRANSFORMED BY: Epstein-Barr
Description: human chronic B cell leukemia established from the peripheral blood of a 73-year-old man with B-prolymphocytic leukemia (PLL) at diagnosis; cell line was established by EBV-transformation during treatment with phorbol ester TPA; cells express mRNA of the proto-oncogenes bcl-2 and bcl-3 confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. E. Matutes, Royal Marsden Hospital, London, UK
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.1-0.5 x 106 cells/ml; seed out at ca. 0.5 x 106 cells/ml at 37C with 5% CO2 maximum density at 1.0-1.5 x 106 cells/ml;
doubling time of ca. 30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (near tetraploid without clear mode; 87-93<4n>XXYY, +3, -7, -8, -10, +12, +12, -14, -19, -19, -20, -20; appears to be derivate of original hyperdiploid line which exhibited trisomy 12) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13- CD19+ CD37+ HLA-DR+ s/cyIgM+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP)
- Clin Exp Immunol 1988;73:23-28 - PMID: 3262465
- Int J Cancer 1986;38:531-538 - PMID: 3093393
- J Clin Pathol 1985;38:897-903 - PMID: 2993372
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.