This is the complete description of the cell line that you selected.
By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, chronic, B cell
DSMZ catalog code: ACC 12
Morphology: lymphoblastoid, round to polygonal cells growing in clusters in suspension
TRANSFORMED BY: Epstein-Barr
Description: human chronic B cell leukemia established from the peripheral blood of a 63-year-old woman with B-prolymphocytic leukemia (B-PLL) at diagnosis; cell line was established by EBV-transformation during treatment with phorbol ester TPA; cells were described to have immunoglobulin light chain restriction (lambda); cells express mRNA of proto-oncogene bcl-2 confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. E. Matutes, Royal Marsden Hospital, London, UK
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.1-0.5 x 106 cells/ml; cells might grow slowly; split 1:2 to 1:4 every 2-4 days at 37C with 5% CO2 maximal density at about 1.5 x 106 cells/ml;
doubling time of ca. 50-70 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (pseudodiploid; 46(42-46)<2n>XX, +3, -8, del(1)(q42), der(3)t(3;8)(p13;q12), der(14)t(11;14)(q13;q32)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13- CD19+ CD37+ HLA-DR+ sIgM+) * isoenzymes (confirmed as human with IEF of MDH, NP) * reverse transcriptase (not detected (RDDP assay))
- Clin Exp Immunol 1988;73:23-28 - PMID: 3262465
- Int J Cancer 1986;38:531-538 - PMID: 3093393
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.