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TUMOR: leukemia, T cell
ECACC catalog code: 88042803
Description: Human leukaemic T cell lymphoblast
Viruses: See Properties
Depositor: Prof H Harris/Dr C Madin, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Products: Interleukin 2 (IL-2), human alpha interferon Applications: IL-2 assay and cloning studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Karyotype: Pseudodiploid, modal No 46
Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41C for 48h followed by a limiting dilution cloning over macrophages.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
- J Immunol 1984;133:123 - PMID: 6327821
- J Immunol Methods 1993;157:203-207 - PMID: 8423364
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.