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TISSUE/ORGAN: pituitary gland
TUMOR: pituitary tumor
ECACC catalog code: 94050406
Transfected by: Transfection Back To Transfection WebPage
Description: Mouse pituitary tumour
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transfection studies, i.e.. endocrine and exocrine secretory pathways.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Horse Serum (HS). Split confluent cultures 1:3 to 1:5 using 0.25% trypsin/EDTA; 15% CO2; 37C. Cell clumps must be broken up to prevent cells growing in suspension.
This F2 subclone was developed from original AtT-20, an ACTH secreting cell line established from murine pituitary tumour. These cells are readily transfected using a standard calcium phosphate protocol.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1795
- Cell 1982;28:51 - PMID: 6279313
- ibid 1983;35:531
- J Cell Biol 1985;101:639 - PMID: 2991303
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.