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TUMOR: leukemia, T cell
ECACC catalog code: 93031145
TRANSFORMED BY: Radiant Agent Back To Radiant Agents' WebPage
Description: Human acute T cell leukaemia
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: TcR, CD2, CD28, CD45 (low) Products: Interleukin 2 (IL-2) in response to calcium ionophores and phorbol ester
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Derived from Jurkat E6-1 clone after gamma irradiation and complement-mediated lysis with anti CD45. Low expression of CD45 (5-8% compared to parent line) results in defective signal transduction through TcR and the accessory molecule, CD2. Interleukin 2 (IL-2) is produced in response to calcium ionophore and phorbol ester stimulation, but it does not produce IL-2 in response to TcR or CD2 stimulation.
Research council deposit: No;
Price Code: C;
Validated by: immunological analysis (TcR+ CD2+ CD28+ (CD45+ only 5-8%)) * isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1990
- Proc Natl Acad Sci U S A 1991;88:2037-2041
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.