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ECACC catalog code: 94101452
Description: Rat dorsal prostatic adenocarcinoma
Viruses: See Properties
Depositor: Dr J Isaacs, Johns Hopkins Oncology Centre, Baltimore MD, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Used in studies in which micro-cell mediated chromosomal transfer of human chromosome 11 suppressed metastatic ability. This suppresser function was localized to 11p11.2-13. (Cancer Research 1992).
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: 44XY, +4, + 12 del(3)(3q32-36)del(15)p14
A highly metastatic, anaplastic, androgen-independent sub-line which spontaneously developed during the serial passage of the non-metastatic, well-differentiated, androgen-responsive R-3327-H sub-lines in the laboratory of Dr J Schalken, Nijmegan, The Netherlands.
Research council deposit: No;
Price Code: C;
- Cancer Res 1992;52:3486 - PMID: 1596907
- Prostate 1988;13:165-188 - PMID: 3174494
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.