This is the complete description of the cell line that you selected.
By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).
TUMOR: leukemia, monocytic
ECACC catalog code: 93120824
Susceptibility to viruses: adenovirus 3 * poliovirus 1 * vesic. stomatitis (Indiana)
Description: Human monocytic leukaemia, HeLa characteristics
Viruses: See Properties
Depositor: Dr M Ferrari, Instituto Zooprofilattico, Brescia, ITALY
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 46
Derived from a human monocytic leukaemia. The cells support the growth of poliovirus I, adenovirus 3 and VSV (Indiana strain) This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: No;
Price Code: C;
Validated by: DNA fingerprinting (HeLa profile) * isoenzymes (species confirmation)
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.