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ECACC catalog code: 94101451
Description: Rat dorsal prostatic adenocarcinoma
Viruses: See Properties
Depositor: Dr J Isaacs, Johns Hopkins Oncology Centre, Baltimore MD, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Carcinogenesis studies, cytogenetic analysis.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:10 to 1:20 i.e. seeding at 2-4 x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
AT-3.1 is a subclone of the AT-3 cell line being one of a series of related variants originating from a single spontaneous adenocarcinoma in the dorsal lobe of the prostate of an aged male inbred Copenhagen rat. AT-3.1 was derived from an anaplastic tumour with a high rate of metastases and is androgen-independent.
Research council deposit: No;
Price Code: C;
- Prostate 1988;13:165-188 - PMID: 3174494
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.