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ICLC catalog code: HTL97010
Continuous culture, grown as suspension
round cells, clusters morphology
SPECIES: human (male)
TUMOR: carcinoma, small cell
DEPOSITOR: Dr S. Ferrini, Immunopharmacology Dept, IST at ABC, Genoa, Italy.
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: negative for HLA antigens
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10-20% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 2 - 4 x 10^5 cells/ml; split cultures 1:2-1:3; 37C, 5% CO2. Passage: 67, mycoplasma negative, HOECHST and PCR
After thawing, the cells appear very suffering, and grow slowly (thaw with medium supplemented with FBS 20%)
Validated by: isoenzymes (confirmed as human with MD, NP.)
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.