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SPECIES: lepidoptera sciaridae diptera - Spodoptera frugiperda (fall armyworm)
TISSUE/ORGAN: pupal ovary
DSMZ catalog code: ACC 119
Morphology: 90% round cells; 10% spindle-shaped, adherent
Susceptibility to viruses: baculovirus
Description: insect - fall armyworm (Spodoptera frugiperda) this cell line (full name: IPLB-SF-21-AE) was derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae (species Spodoptera frugiperda, family Sciaridae, order Lepidoptera); cells are susceptible to Baculovirus infection; see also SF-9 (DSM ACC 125) which was derived from SF-21 confirmed with IEF of AST, PEP B as not primate or murine
Depositor: Dr. V. Jaeger, GBF, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% TC 100 medium + 10% FBS split culture 1:3 to 1:5 every 4-5 days; cells grow partly adherent, but the use of trypsin is not necessary, detach cells with rubber scraper at about 20C (room temperature) without CO2 (keep lid of flask closed) about 25 x 106 cells/80 cm 2;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
- In Vitro 1977;13:213-217 - PMID: 68913
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.