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TISSUE/ORGAN: skin, fibroblast
ECACC catalog code: 90112813
Susceptibility to viruses: herpes simplex * vaccinia * vesic. stomatitis (Indiana)
Description: Muntjac skin
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Chromosome studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F10 + 2mM Glutamine + 20% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 7
Derived from a skin biopsy of an adult male muntjac. The cells have a diploid chromosome number of 7 in the male and 6 in the female, amongst the lowest determined for a mammalian species. Recent tests indicate the cell line is positive for BVDV, both for antigen and infectious virus. Susceptible to vesicular stomatitis (Indiana strain), herpes simplex and vaccinia viruses.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 157
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.