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SPECIES: human, Caucasian
DSMZ catalog code: ACC 165
Morphology: fibroblast-like adherent cells growing as monolayer
Susceptibility to viruses: adenovirus 12 * Coxsackie B3 * herpes simplex * poliovirus 3 * vaccinia * vesic. stomatitis (Indiana)
Description: human neuroblastoma established from an abdominal mass (diagnosed as neuroblastoma) of a 1-year-old Caucasian boy in 1967 confirmed as human with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS + 1x non-essential amino acids split confluent culture 1:2 every 3-5 days (trypsin should not be used, cells can be detached by tapping the flask or pipetting); seed out at ca. 0.5-1.0 x 106 cells/cm 2 at 37C with 5% CO2 about 1-2 x 106 cells/ml or 0.5 x 106 cells/cm 2;
doubling time of ca. 40-50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hyperdiploid karyotype; 49(44-51)<2n>XY, +1, +6, +12, der(1)t(1;2HSR)(p33/35;?p22)x2, der(16)t(2;16)(p21/22;q24/25); HSR regions probably represent amplification of the N-myc oncogene which maps to 2p22 involved in the t(2;16) als) * immunological analysis (cytokeratin- desmin- endothel- GFAP- neurofilament+ vimentin++) * isoenzymes (confirmed as human with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CCL 127, ICLC: HTL96021
- Cancer Res 1970;30:2110 - PMID: 5459762
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.