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SPECIES: rat, Wistar
ECACC catalog code: 87050701
Description: Rat Wistar liver hepatoma
Viruses: See Properties
Depositor: Dr G Williams, Haylor Dana Institute for Disease Prevention, New York, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Carcinogenesis and tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cells 1:3 to 1:6 i.e. 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derived from the liver of a Wistar rat and is useful in quantitative carcinogenesis studies. Inoculation into new born rats is more sensitive than nude mice for detection of tumorigenicity of rat liver lines.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Cancer Res 1979;39:4441 - PMID: 40692
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.