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DSMZ catalog code: ACC 152
Morphology: epithelial-like cells growing in monolayers
Description: porcine normal kidney cells derived as a subclone from the cell line AM II which was established from a normal porcine kidney in 1983; cells have been used in assays to test toxins and mutagens
Viruses: ELISA. reverse transcriptase positive
Depositor: Dr. K. E. von Milczewski, Bundesanstalt fuer Milchforschung, Institut fuer Mikrobiologie, Kiel, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-90% Medium 199 (Hank's salts) + 10-20% FBS cells are difficult to culture and grow slowly to very slowly! split confluent culture 1:5 to 1:10 about once a week using trypsin/EDTA at 37C with 5% CO2 cell harvest of about 8 x 106 cells/80 cm 2 or 1 x 107 cells/175 cm 2;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
- Mycotoxin Res 1987;3:69-76
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.