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SPECIES: human, Caucasian
TISSUE/ORGAN: bone marrow
TUMOR: multiple myeloma
ECACC catalog code: 86051302
Description: Human B lymphocyte
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Immunoglobulin A (IgA) Applications: Biosynthetic and surface marker studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 2-4x100,000 cells/ml; 5% CO2; 37C. Maximum cell density 1,000,000 cells/ml.
Karyotype: 2n = 46
Derived from bone marrow removed from a female patient with multiple myeloma. The cells synthesise IgG at a rate of 2.4ug/1,000,000 cells/day and have receptors for insulin, calcitonin and human growth hormone. They also exhibit surface markers and receptor sites characterised of B lymphocytes.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 159
- Ann N Y Acad Sci 1972;190:221-234
- J Biol Chem 1974;249:1661-1667 - PMID: 4361817
- Nature 1974;251:443-444 - PMID: 4608784
- Proc Natl Acad Sci U S A 1974;71:84-88 - PMID: 4359334
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.