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SPECIES: mouse, AKR
ECACC catalog code: 94031011
TRANSFORMED BY: Radiant Agent Back To Radiant Agents' WebPage
Description: Mouse, AKR, thymoma
Viruses: See Properties
Depositor: Dr D Iannelli, University of Naples, School of Agriculture, ITALY (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Study of the assembly and transport of Ly-2/3 antigen in the cell surface or to define the regions of Ly-2/3 interacting with the corresponding antibodies and with MHC class I molecules.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 10% Horse Serum (HS). Maintain cultures between 3-9x100,000 cells/ml; 5-10% CO2; 37C.
The parent cell line AKR/1a murine T cell line was exposed to a dose of 500 rads from an X-ray source. The mutant Lyt2- cells were selected out by magnetic bead separation.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ICLC: ATL98012
- Immunogenetics 1994;40:151
- J Immunol Methods 1992;154:211 - PMID: 1401954
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.