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SPECIES: rat, DB1X
DSMZ catalog code: ACC 132
Morphology: large, relatively broad, adherent cells growing in monolayers
Description: rat smooth muscle established from the thoracic aorta of a DB1X embryonic rat in 1976; cells were described to display properties characteristic of smooth muscle cells confirmed as rat with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase positive
Depositor: obtained from ATCC (CRL 1476), Rockville, Maryland, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% Dulbecco's MEM + 20% FBS cell growth is very slow! split confluent culture 1:2 to 1:3 once a week using trypsin/EDTA; seed out at ca. 0.5-0.8 x 104 cells/cm 2 at 37C with 5-10% CO2 about 1-2 x 106 cells/80 cm 2;
doubling time of ca. 50-100 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 105 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * isoenzymes (confirmed as rat with IEF of AST, MDH, NP) * reverse transcriptase (negative)
Availability in cell line catalogues: ATCC: CRL 1476
- Exp Cell Res 1976;98:349-366 - PMID: 943301
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.