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TISSUE/ORGAN: intestine, small
DSMZ catalog code: ACC 111
Morphology: adherent, transparent, epitheloid cells growing as monolayers (polymorphy of cell forms)
Description: rat normal small intestine normal rat epithelial cells from the small intestine; cells were described to synthesize fibronectin and collagen; no continuous cell line: about 10-15 doublings after thawing of the stock cells confirmed as rat with IEF of AST, MDH, MPI, NP
Viruses: ELISA: reverse transcriptase negative
Depositor: obtained from ATCC (CRL 1592), Rockville, Maryland, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
45% Dulbecco's MEM (4.5 g/L glucose) and 45% RPMI 1640 + 10% FBS + 0.1 U/ml insulin cells are difficult to culture! split confluent cultures 1:2 about twice a week using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm 2 at 37C with 5% CO2 about 16 x 106 cells/175 cm 2;
doubling time of about 50 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: isoenzymes (confirmed as rat with IEF of AST, MDH, MPI, NP)
Availability in cell line catalogues: ATCC: CRL 1592, IZSBS: BS CL 117
- J Cell Biol 1979;80:248-265 - PMID: 88453
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.