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SPECIES: grass frog
ECACC catalog code: 89072615
Morphology: Fibroblast like
Description: Frog Grass embryo
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Genetic studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
L-15 (50%) + Distilled Water (40%) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 25C. Do not use CO2.
Karyotype: 2n = 26, Stable haploid
Previously designated RPH68.2A. Derived from androgenetic haploid embryos of the grass or leopard frog, (Rana pipiens). The cells retain their haploid chromosome complement for over 200 generations. Used in genetic studies.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 145
- Proc Natl Acad Sci U S A 1970;65:337 - PMID: 5263768
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.