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SPECIES: rat, Sprague-Dawley
DSMZ catalog code: ACC 208
Morphology: grows as single cells in suspension
Description: rat hepatoma established from the hepatoma of a 16-month-old SD-female rat in 1988 confirmed as rat with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative
Depositor: obtained from DKFZ Tumorbank, Heidelberg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS optimal split ratio of 1:2 to 1:3 twice per week at 37C with 5% CO2 cell harvest of about 0.6 x 106 cells/ml;
doubling time of about 29 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hypodiploid rat karyotype with 12% tetraploidy; 38(35- 41)<2n>; 2 large submetacentric and 1 large metacentric centric fusion markers; sdl 30-32/60-62 present at 14%) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * isoenzymes (confirmed as rat with IEF of MDH, NP) * reverse transcriptase (reverse transcriptase not detected)
- Biochem Cell Biol 1988;66:1-12 - PMID: 3370139
- Verh Dtsch Ges Inn Med 1979;85:1296-1300
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.