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SPECIES: mouse, BALB/cJ
TISSUE/ORGAN: testis, Leydig cells
TUMOR: testicular tumor
ECACC catalog code: 89031605
Susceptibility to viruses: herpes simplex * pseudorabies * vaccinia * vesic. stomatitis (Indiana)
Description: Mouse BALB/c Leydig cell testicular tumour
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Steroids Applications: Tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Karyotype: 2n = 40, hyperdiploid
Derived from a Leydig cell testicular tumour of a BALB/c mouse. The cells secrete steroids; this increases at saturation density. Secretion should be retained for over 50 passages. Exposure to 0.00001M cAMP stimulates secretion 4-5 fold. The major products are progesterone and its derivative 20 alpha-hydroxy-4-peugnen-3-one. It does not respond to interstitial cell stimulating hormone (ICSH) but is stimulated by cyclic adenosine monophosphate (AMP).
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 83
- Endocrinology 1967;81:440 - PMID: 4382264
- Science 1966;154:1186 - PMID: 4288399
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.