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SPECIES: human, Japanese
DSMZ catalog code: ACC 223
Morphology: epitheloid cells growing adherently in monolayers
Description: human pancreas carcinoma established from the malignant ascites of a 60-year-old Japanese man with pancreas carcinoma in 1985 confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. S. Saito, Toyama Medical and Pharmaceutical University, Toyama, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% MEM (with Earle's salts) + 20% FBS + 1% non-essential amino acids + 1% sodium pyruvate optimal split ratio of 1:2 to 1:3 every 2-5 days using trypsin/EDTA at 37C with 5% CO2 cell harvest of about 1-2 x 105 cells/cm 2;
doubling time of about 38 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near-triploid karyotype with 11% polyploidy and 1-2 supernumerary dm; 69/70(66-73)<3n>X, ?del(X)/XY, -Y, -2, -4, +6, +7, +8, - 9, -9, +11, +16, +mar; inv(3)(p22q24), i(8q), idic(9)(pter), add(17)(p12); 2 medium size ring ch prese t at 10%) * immunological analysis (cytokeratin + (100%), desmin -, endothel -, GFAP -, neurofilament -, vimentin + (1-5%)) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase not detected)
Availability in cell line catalogues: ECACC: 93121056, ICLC: HTL95006
- Int J Pancreatol 1993;13(1):31-41 - PMID: 8454916
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.