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SPECIES: human, Japanese
DSMZ catalog code: ACC 259
Morphology: epitheloid cells growing adherently in monolayers and clusters
Description: human pancreas carcinoma established from the malignant ascites of a 66-year-old Japanese man with pancreas carcinoma in 1984 confirmed as human with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. S. Saito, Toyama Medical and Pharmaceutical University, Toyama, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% MEM (with Earle's salts) + 10% FBS + 1% non-essential amino acids + 1% sodium pyruvate split confluent culture 1:10 once a week using trypsin/EDTA; seed out about 1 x 106 cells/175 cm 2 at 37C with 5% CO2 about 14 x 106 cells/175 cm 2;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1.4 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (flat-moded hypodiploid karyotype with 13% polyploidy; 39(36-40)<2n>XY, -6, -8, -9, -10, -12, -13, -17, -17, -19, -20, +3 mar (2 rings), del(4)(p15), add(11)(q22.3;HSR), add(12)(q24;HSR), add(19)(p13), add(21)(p12); gene amplification) * cytogenetics 2 (suggested by large HSR; ch17 nullisomy) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ (100%), desmin-, endothel-, GFAP-, neurofilament-, vimentin-) * isoenzymes (confirmed as human with IEF of AST, NP) * reverse transcriptase (reverse transcriptase not detected)
Availability in cell line catalogues: ECACC: 93121055, ICLC: HTL96006
- Int J Pancreatol 1993;13(1):31-41 - PMID: 8454916
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.