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ECACC catalog code: 95091410
Description: Human cervix carcinoma, HeLa derivative, transformed
Viruses: See Properties
Depositor: Prof H Bujard, ZMBH, Heidelberg, GERMANY (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Gene activation and expression studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:5 to 1:20 i.e. seeding at 2x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
The HtTA-1 cell line was derived from HeLa cells being stably transfected with a tetracycline-controlled transactivator (tTA) consisting out of the tet repressor fused with the activating domain of virion protein 16 of herpes simplex virus. This transactivator stimulates transcription from a minimal promoter sequence derived from the human cytomegalovirus promoter IE combined with tet operator sequences. This system allows differential control of the activity of an individual gene in mammalian cells and is suitable for creation of 'on/off' situations for such genes in a reversible way.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Proc Natl Acad Sci U S A 1992;89:5547
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Barbara Parodi
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.