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SPECIES: rat, buffalo
ECACC catalog code: 93120108
Description: Rat hepatoma
Viruses: See Properties
Depositor: Dr M Simpkins/Dr H Harris, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Dexamethasone phosphate; hydrocortisone Applications: Tumourigenicity and enzymatic studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 66, hypertriploid
Derived from a rat ascites tumour, which in turn was derived from a solid hepatoma induced in male Buffalo rats by feeding them 0.04% N,N'-2,7fluorenyle-bis-2,2,2-trifluoroacetamide for 12.4 months.
Research council deposit: Yes;
Price Code: B;
Validated by: DNA fingerprinting * isoenzymes ((species confirmation))
Availability in cell line catalogues: ICLC: ATL95006
- Adv Cancer Res 1965;9:227 - PMID: 4285261
- ATLA 1988;16:32
- Proc Natl Acad Sci U S A 1966;56:269 - PMID: 4381784
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.