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Monoclonal continuous culture, grown as suspension
SPECIES: rat, buffalo (male) Karyology: extra chromosomes 1 and 13
TISSUE/ORGAN: ascitic fluid
tumorigenic in syngeneic animals
Cell line available under some conditions.
PROPERTIES: biochemical markers; enzymology; toxicity screening; tumorigenicity; cytotoxicity; xenobiotic metabolism; dexamethasone p receptor
Available in the following LABORATORY:
- Istituto Patologia Generale, Universita' degli Studi (GEUCC, Genova)
MEM + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-30, mycoplasma negative, HOECHST
Growth also as monolayer. Derived from hepatoma induced by N,N
Validated by: beuraldehyde dehydrogenase (high levels) * chromosome analysis (large metacentric chromosome 1)
Availability in cell line catalogues: ECACC: 93120108, ICLC: ATL95006
- Adv Cancer Res 1965;9:227 - PMID: 4285261
- ATLA 1988;16:32
- Proc Natl Acad Sci U S A 1966;56:269 - PMID: 4381784
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.