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ECACC catalog code: 85061109
Description: Human Caucasian colon carcinoma
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 46, hypertriploid
Subclone of parent HT-29 which was established from adenocarcinoma from a 44-year-old female Caucasian. Tumorigenic in nude mice and has ability to grow in agar.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
- Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
- J Natl Cancer Inst 1977;58:1743 - PMID: 864752
- Proc Soc Exp Biol Med 1981;166:107 - PMID: 7465568
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.