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SPECIES: human, Caucasian
ECACC catalog code: 87012701
Description: Human Caucasian plasma cell plasmacytoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Immunoglobulin G (IgG) kappa chains in spinner culture. Endoplasmic reticulum in stationary culture. Applications: Tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C. A density of 3x1,000,000 cells/ml may be achieved.
Karyotype: 2n = 46, hyperdiploid
Derived from a plasmacytoma of a 56 year old with IgG multiple myeloma. They act as true plasma cells, developing endoplasmic reticulum when grown as stationary suspension cultures. In spinner culture the cells produce IgG kappa chains. Is EBNA positive.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1484
- J Immunol 1976;118:1480
- Nature 1974;250:507 - PMID: 4549735
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.