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SPECIES: dog, golden retriever
TUMOR: not defined tumor
ECACC catalog code: 89050908
Susceptibility to viruses: canine adenovirus * canine coronavirus * canine herpes * canine parainfluenza * canine parvovirus
Description: Canine Golden Retriever tumour
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: Canine corona-, Ad I and II, parainfluenza, herpes and parvo- viruses
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
L-15 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 0.02% EDTA; 37C. CO2 is detrimental; free gas exchange with atmospheric air necessary.
Derived from tumour of thigh (unknown histology) of 8 year old Golden Retriever which had a previous history of mammary adenocarcinoma. Susceptible to canine corona-, canine Ad type I and II, canine herpes, canine parainfluenza and canine parvo- viruses.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1542
- Am J Vet Res 1980;41:55 - PMID: 7362124
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.