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SPECIES: human, Caucasian
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, promyelocytic
ECACC catalog code: 88120805
Description: Human Caucasian promyelocytic leukaemia
Viruses: See Properties
Depositor: Dr G Brown/Dr C M Bunce, Immunology Department The Medical School, University of Birmingham, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO). Maintain cultures between 2x100,000 to 1,000,000 cells/ml. NB: The cells will differentiate if the culture medium is allowed to become acidic. 5% CO2; 37C. Freeze in 10% glycerol and 90% FBS.
HL60 15-12 is a variant of HL60 which is capable of differentiating towards either neutrophils or monocytes with treatment of appropriate agents.
Research council deposit: Yes;
Price Code: B;
Validated by: isoenzymes (species confirmation)
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.