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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute myeloid
DSMZ catalog code: ACC 3
Morphology: round, single cells in suspension
Susceptibility to viruses: HIV-1 * HTLV-1
Description: human acute myeloid leukemia established from the peripheral blood of a 35-year-old woman with acute myeloid leukemia (AML FAB M2) in 1976; cells can be used for induction of differentiation studies; described to be responsive to DMSO, phorbol ester TPA and other reagents and to carry amplified c-myc gene; present cells are apparently tetraploid derivates of hypodiploid original where c-myc was amplified in dmin (instead of hsr) confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. E. Porfiri, The Royal Free Hospital, Department of Haematology, London, UK
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.0 x 106 cells/ml, split 1:2 to 1:5 every 1-2 days; seed out at about 1 x 106 cells/ml at 37C with 5% CO2 maximal density of 1.5-2.0 x 106 cells/ml;
doubling time of about 25 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotetraploid karyotype without clear mode, 1.5% polyploidy; 82(78-88)<4n>XX, -X, -X, -2, -3, -4, -5, -8, -9, -10, -14, -16, -17, -17, +3m, der(6)t(6;?)(q25;?)/dup(6)(q23;qter)x2, del(9)(p22), del(11)(q22/23), der(16)t(16;17)) * cytogenetics 2 ((q22/23;q21-22)x2; high frequency of subclonal ch 1 rearrangements) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13+ CD14- CD15+ CD19- CD33+ HLA-DR-) * isoenzymes (confirmed as human with IEF of MDH, NP) * reverse transcriptase (not detected (RDDP assay))
Availability in cell line catalogues: ATCC: CCL 240, ICLC: HTL95010
- Blood 1979;54:713-733 - PMID: 288488
- Blood 1988;71:242-247 - PMID: 3422031
- Nature 1977;270:347-349 - PMID: 271272
- Proc Natl Acad Sci U S A 1978;75:2458-2462 - PMID: 276884
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.