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DSMZ catalog code: ACC 81
Morphology: adherent growth in monolayers, mostly spindle-like, some cells with cellular processes, 1-5% very big cells, no strict contact inhibition
Description: human melanoma established from malignant melanoma confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. G. E. Moore, Colorado Oncology Foundation, Denver, Colorado, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture about 1:6 once a week using trypsin/EDTA; seed out at about 1 x 106 cells/ml at 37C with 5% CO2 cell harvest of about 8 x 106 cells/175 cm 2;
doubling time of about 60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP)
Availability in cell line catalogues: ATCC: HTB 45
- J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
- Oncogene 1994;9(3):939-942 - PMID: 8108138
- Proc Natl Acad Sci U S A 1987;84(22):7972-7976 - PMID: 2825172
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.