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SPECIES: mouse, C57L
DSMZ catalog code: ACC 175
Morphology: adherent, epithelial cells growing as monolayer
Description: mouse hepatoma cell line was derived from the BW7756 tumor that arose in a C57L mouse; cells were described to secrete several liver-specific products (e.g. albumin, alpha1-antitrypsin, alpha-fetoprotein, amylase); cells may grow in serum-free medium when supplemented with dexamethasone and selenium (= ATCC CRL 1830) confirmed as mouse with IEF of AST, MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM (4.5 g/L glucose) + 10% FBS split confluent culture 1:4 to 1:10 every 3-6 days using trypsin/EDTA; cells grow rather quickly; seed out at ca. 1-2 x 106 cells/25 cm 2 at 37C with 5-10% CO2 about 10-15 x 106 cells/80 cm 2;
doubling time of about 18 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotetraploid karyotype with centric fusion and 6% polyploidy; flat-moded, 53-70<4n>) * isoenzymes (confirmed as mouse with IEF of AST, MDH, NP, PEP B) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: CRL 1830, ECACC: 92110305, IZSBS: BS TCL 157
- J Natl Cancer Inst 1980;64:809-819 - PMID: 6102619
- Methods Enzymol 1987;151:19-38 - PMID: 3431441
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.