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TUMOR: carcinoma, hepatocellular
DSMZ catalog code: ACC 180
Morphology: adherent, epithelial-like cells growing as monolayers and in small aggregates
Description: human hepatocellular carcinoma established from the tumor tissue of a 15-year-old Argentine boy with hepatocellular carcinoma in 1975; cell line is patented; cells were described to not harbor a hepatitis B virus genome; cells reportedly produce a variety of proteins: alpha-fetoprotein, albumin, alpha2-macroglobulin, alpha1-antitrypsin, transferrin, alpha1-antichymotrypsin, haptoglobin, ceruloplasmin, plasminogen, complement (C3, C4), C3 activator, fibrinogen, alpha1-acid glycoprotein, alpha2-HS glycoprotein, ss-lipoprotein, retinol binding protein confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: obtained from ATCC (HB 8065), Rockville, Maryland, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS split confluent culture 1:2 to 1:4 every 3-6 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/25 cm 2 ; after thawing, initially to be cultured with 20% FBS at 37C with 5% CO2 cell harvest of about 25-30 x 106 cells/80 cm 2 ,;
doubling time of ca. 50-60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hyperdiploid karyotype; 52(47-54)<2n>XY, +2, +14, +17, +20, +2m, t(1;21)(p22.2;p11/12), i(17q)/ der(17)t(17;17)(p11;q11)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin ++ (100%) desmin- endothel- GFAP- neurofilament- vimentin-) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: HB 8065
- In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
- J Lipid Res 1986;27:858 - PMID: 3021884
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.