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SPECIES: human, Caucasian
TUMOR: carcinoma, hepatocellular
ECACC catalog code: 85011430
Description: Human Caucasian hepatocyte carcinoma
Viruses: See Properties
Depositor: Prof B Knowles, Wistar Institute, Philadelphia, USA
No restrictions. Patent: This material is cited in a US and/or other Patent and may not be used to infringe parent claims.
Properties: Products: Prothrombin, antithrombin III, alpha-foetoprotein complement, C3 and C4 activator, fibrinogen Applications: Study of hepatocyte function and specific protein expression
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No 55
The Hep G2 cell line has been isolated from a liver biopsy of a male Caucasian aged 15 years, with a well differentiated hepatocellular carcinoma. The cells secrete a variety of major plasma proteins e.g.. albumin, alpha2-macroglobulin, alpha 1-antitrypsin, transferrin and plasminogen. They have been grown successfully in large scale cultivation systems. Hepatitis B virus surface antigens have not been detected. Hep G2 cells have been shown to be G418 resistant (400µg/ml). The cells will respond to stimulation with human growth hormone.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ICLC: HTL95005
- In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
- J Lipid Res 1986;27:858 - PMID: 3021884
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.