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SPECIES: human, Black
TUMOR: carcinoma, hepatocellular
DSMZ catalog code: ACC 93
Morphology: epithelial-like cells growing as monolayers
Description: human hepatocellular carcinoma established from the tumor tissue of an 8-year-old black boy in 1976; description: cells contain integrated hepatitis B virus genome; cells were described to produce a variety of proteins, e.g. alpha-fetoprotein, albumin, transferrin, alpha2-macroglobulin, alpha1-antitrypsin, haptoglobin and others; patented cell line; this cell line has been assigned by the ZKBS (German Central Commission for Biological Safety) to risk category 2 confirmed as human with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. H. J. Gruss, Medical School of Freiburg, Freiburg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% MEM (with Earle's salts) + 10% FBS + 2 mM L-glutamine split confluent culture 1:4 to 1:10 every 3-5 days using trypsin/EDTA at 37C with 5% CO2 about 5 x 106 cells/80 cm 2;
frozen with 70% medium, 20% FBS, 10% DMSO at 1 x10 6 cells/ampoule;
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (hypotriploid stemline with near tetraploid side line (6%); 63(60-64)<3n>XX, -1, -1, -2, -3, -4, -8, -9, -13, -Y, +der(11)t(1;11)(q11;q14)x2, +der(?15)t(1;?15)(q11;q24), i(7p)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase positive)
Availability in cell line catalogues: ATCC: HB 8064
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.