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SPECIES: human, Black
TUMOR: carcinoma, hepatocellular
ECACC catalog code: 86062703
Description: Human Negroid hepatocyte carcinoma
Viruses: See Properties
Depositor: Mr C B Morris, EMBL, Heidelburg, GERMANY
No restrictions. Patent: This material is cited in a US and/or other Patent and may not be used to infringe parent claims.
Properties: Products: Alpha-foetoproteins, Hepatitis B Surface Antigen (HBsAG), albumin, transferrin, fibrinogen, complement (C3), alpha-2-macroglobulins
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 46, modal No 60
Derived from 8-year-old Negro male. Cells contain integrated Hepatitis B virus genome. However there is currently no evidence that this cell line produces infectious Hepatitis B virus. The cells should be handled under laboratory containment level 2.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: HB 8064
- Nature 1979;282:615-616 - PMID: 233137
- Science 1980;209:497-499 - PMID: 6248960
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.