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ICLC catalog code: HTL95020
Continuous culture, grown as semiadherent
SPECIES: human, Black (female, 31 years old) Karyology: modal number 68
TUMOR: carcinoma, epitheloid
Susceptibility to viruses: adenovirus 5 * poliovirus 1 * vesic. stomatitis (Indiana)
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: toxicity screening; virology; bacterial adhesiveness; bacterial invasiveness; enzymology; tumorigenicity
Available in the following LABORATORY:
- IRCCS AOU San Martino -IST Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
Ham's F12 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Semiadherent cell line, adaptable to growth in suspension (spinner) culture. Maintain culture between 3 and 9x105 cells/ml; split 1:2-1:4. 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with AST, G6PD, MD, NP, PepB)
Availability in cell line catalogues: ECACC: 87110901
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.