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SPECIES: human, Black
TUMOR: carcinoma, epitheloid
DSMZ catalog code: ACC 57
Morphology: epithelial-like cells growing in monolayers
Susceptibility to viruses: adenovirus 3 * arbovirus * Coxsackie * echovirus * measles * poliovirus 1 * reovirus 3 * respiratory syncytial virus * rhinovirus * vaccinia
Description: human cervix carcinoma established from the epitheloid cervix carcinoma of a 31-year-old black woman in 1951; later diagnosis changed to adenocarcinoma; first aneuploid, continuously cultured human cell line confirmed as human with IEF of G6PD, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: obtained from ATCC (CCL 2), Rockville, Maryland, USA
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% MEM (with Earle's salts) + 10% FBS + 2 mM L-glutamine + non-essential amino acids (cells also grow well in 90-95% RPMI 1640 + 5-10% FBS) split confluent culture 1:4 to 1:6 every 3-5 days using trypsin/EDTA; cells reach confluence quickly; seed out at ca. 1-2 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of ca. 8-15 x 106 cells/80 cm 2;
doubling time of ca. 48 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of GPDH, MDH, NP) * reverse transcriptase (reverse trancriptase not detected)
Availability in cell line catalogues: ATCC: CCL 2, IZSBS: BS TCL20, ECACC: 93021013, ICLC: HTL95023
- Cancer Res 1952;12:264
- J Exp Med 1953;97:695
- J Gen Virol 1983;64:825 - PMID: 6834007
- J Virol 1978;28:444 - PMID: 214572
- Obstet Gynecol 1971;38:945-949 - PMID: 4942173
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.