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SPECIES: human, Black
TUMOR: carcinoma, epitheloid
ECACC catalog code: 93021013
Susceptibility to viruses: adenovirus 3 * arbovirus * Coxsackie * echovirus * measles * poliovirus 1 * reovirus 3 * respiratory syncytial virus * rhinovirus * vaccinia
Description: Human Negroid cervix epitheloid carcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1.3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 46, aneuploid
Derived from a cervical carcinoma from a 31 year old Negro. This was the first aneuploid line derived from human tissue maintained in continuous cell culture. Susceptible to Poliovirus type I and adenovirus type 3. Identified as a contaminant in many other cell lines. The cells should be handled under laboratory containment level 2.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 2, IZSBS: BS TCL20, DSMZ: ACC 57, ICLC: HTL95023
- Cancer Res 1952;12:264
- J Exp Med 1953;97:695
- J Gen Virol 1983;64:825 - PMID: 6834007
- J Virol 1978;28:444 - PMID: 214572
- Obstet Gynecol 1971;38:945-949 - PMID: 4942173
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.