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TUMOR: lymphoma, Hodgkin
DSMZ catalog code: ACC 17
Morphology: single, round to polygonal cells in suspension; heterogeneity in cell size, 15-100 śm or larger; many bi- and polynucleated cells
Description: human Hodgkin's lymphoma established from the pleural effusion of a 74-year-old man with Hodgkin's disease (nodular sclerosing, stage IV) in 1982 confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS cells are difficult to culture! maintain at high density (about 0.5-1.0 x 106 cells/ml); exchange culture medium and add 10-25% new medium; seed out at ca. 1 x 106 cells/ml in a 24-well-plate at 37C with 5% CO2 maximal density of 2.0-2.5 x 106 cells/ml;
doubling time of about 70-75 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 3-5 x 106 cells/ampoule;
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (remarkable human hypodiploid cell line with large number of rearrangements involving all chromosomes except ch 10; 36(35-38)<2n>X, der(Y), breakpoints at: 1p21, 1q11, 2p14, 2q37, 3q21, 3p14, 3q11, 3q25.2, 3q27, 4q25, 5q11, 5q35, q15/16,) * cytogenetics 2 (7p14, 8q21.1, 8q23, 8q24.2, 9p11, 11q13, 12p12, 12q24.3, 13p11, 13q14, 14q21, 14q23/24.2, 15p11, 15q13, 17p11, 17q25, 18p11, 19p13, 19q13, 21q21, 22q33, Ycen, Yq12) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD2+CD3-CD4-CD5-CD6-CD7-CD8-CD13-CD15+CD19-CD30+ HLA-DR+ TCR_/beta-) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase negative)
- Leuk Res 1986;10:487-500 - PMID: 3713248
- Leuk Res 1988;2:371-376
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.