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ECACC catalog code: 91091005
Description: Human colon carcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
McCoy's 5a + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No 45
One of 3 strains of malignant cells isolated from a male with colonic carcinoma. Cells are tumourigenic in nude mice and form colonies on agarose.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 247, IZSBS: BS TCL 100
- Cancer Res 1981;41:1751-1756 - PMID: 7214343
- J Natl Cancer Inst 1982;69:767-771 - PMID: 6956756
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.