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SPECIES: hamster, golden Syrian
DSMZ catalog code: ACC 222
Morphology: epitheloid monolayer
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Description: hamster pancreas carcinoma established from the BHP-induced pancreatic carcinoma of a Syrian golden hamster (GN strain) in 1983 human origin was ruled out with IEF of AST, LDH, MDH, PEP B
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. S. Saito, Toyama Medical and Pharmaceutical University, Toyama, Japan
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS split confluent culture at 1:3 every 3 days or at 1:6 every 5-6 days using trypsin/EDTA at 37C with 5% CO2 cell harvest of about 50-60 x 106 cells/175 cm 2;
doubling time of about 25-30 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near diploid hamster karyotype with tetraploid sideline (18%) and 18% polyploidy; 44(40-45)<2n>X/XX; paucity of homologous chromosomes indicates a high level of rearrangement; hamster markers present) * DNA fingerprinting (unique DNA profile with (gtg)5-probe, heterozygous with human single locus probe pYNH24 probe) * isoenzymes (IEF of AST, LDH, MDH, PEP B could not confirm Syrian hamster as species, but ruled out human species) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ECACC: 93121054
- Gastroenterol Jpn 1988;23:183-194 - PMID: 2838375
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.