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SPECIES: rat, AxC
ECACC catalog code: 94101905
Description: Rat hepatoma
Viruses: See Properties
Depositor: Dr M Weiss, Dept. Biologie Moleculaire, Institute Pasteur, Paris, FRANCE (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Toxicity studies; negative control for differentiated hepatoma
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Coon's modified Ham's F12 + 2mM Glutamine + 5% Fetal Bovine Serum (FBS). Split confluent cultures 1:50 to 1:100 i.e. seeding at 1-2x1,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No 52 (50-55)
H5 is a subclone of H4-II-E-C3 (85061112) which originated from the Reuber H-35 hepatoma induced by chemical carcinogenesis in an A x C rat. The dedifferentiated H5 cells express high levels of glucose-6-phosphate dehydrogenase and exhibit a population doubling time of 12 hours. Differentiated variants are H4IIEC3 (85061112), Fao and others (see Biochimie 1974;56:1603 for details).
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Biochimie 1974;56:1603-1611 - PMID: 4157008
- Cell Growth Differ 1994;5:957 - PMID: 7819133
For information, get in touch with:
IRCCS AOU San Martino IST,
c/o Centro Biotecnologie Avanzate, Torre D - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Assunta Manniello
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.